Batman must face his most ruthless nemesis when a deformed madman calling himself "The Joker" seizes control of Gotham's criminal underworld.

Batman must face The Penguin, a sewer-dwelling gangleader intent on being accepted into Gotham society. Meanwhile, another Gotham resident finds herself transformed into Catwoman and is out for revenge...

In the looming shadows of Gotham City, two villainous souls devise twin schemes of revenge against the city's dark guardian. Two-Face, disfigured by chance and fueled by vengeance, is obsessed with destroying Batman, while the Riddler, a disillusioned genius, pursues the destruction of billionaire Bruce Wayne. Together they've hatched a plot to absorb and control the minds of all of Gotham City's residents, and only Batman and his new partner, Robin, can stop them! But the Dark Knight is hindered by a mystery from his own childhood. He must hope that a new found love can help him overcome the incident that made him what he is. In the end, he must decide: will he be Batman—forever?

Batman and Robin deal with relationship issues while preventing Mr. Freeze and Poison Ivy from attacking Gotham City.