In the fifth series premiere, without the Intersect in his head or the support of the CIA, the world of espionage is a much more dangerous place for Chuck Bartowski and his team of freelance spies. To start, Carmichael Industries takes on a slick, rich thief named Jean Claude (guest star MARK HAMILL - Star Wars), and a conniving investor, Roger Bale (guest star CRAIG KILBORN - The Late Late Show with Craig Kilborn), who has stolen money from some of the world's most dangerous people. Meanwhile, Morgan helps Chuck with a special surprise for Sarah. Back in the Buy More, Jeff and Lester have taken things to another level with their latest scheme.

As the team takes on a missing persons case, Chuck and company struggle to establish themselves as Carmichael Industries in the face of a rival security firm led by the tough and efficient Gertrude Verbanski (recurring guest star CARRIE-ANNE MOSS – The Matrix). Meanwhile, Morgan continues to adjust to the Intersect, while Chuck adjusts to being Morgan’s handler. Also, the Buy More finds an unlikely spokesperson to help motivate business. JEFF FAHEY (Lost, Grindhouse) and JUSTIN HARTLEY (Smallville) also guest star.

As the team is tasked with finding one of the CIA's "most wanted," Chuck fears that life as the Intersect may be going to Morgan's head and inflating his ego. Meanwhile Sarah helps Casey navigate his relationship with Gertrude (recurring guest star CARRIE-ANNE MOSS). Elsewhere, Captain Awesome makes a startling revelation about Jeff and Lester.

Chuck and Sarah go undercover at a Buy More convention to hunt down an assassin whose mission is to take down the new Intersect. Meanwhile, Morgan must deal with the consequences of having the Intersect. At the Buy More, Jeff begins to show a very different side of himself. DAVID KOECHNER (The Office, Anchorman: The Legend of Ron Burgundy) and CATHERINE DENT (The Shield) guest star.

A mission to track down a computer super virus leads Chuck to return to his roots as a hacker - and to call on his biggest competition, Gertrude Verbanksi (recurring guest star CARRIE-ANNE MOSS), for help. Elsewhere, a new Buy More employee (guest star DANNY PUDI – Community) complicates Jeff and Lester's friendship.

Chuck and Sarah race against time to take down a dangerous computer virus, pitting them against Robyn Cunnings (guest star REBECCA ROMIJN – X-Men, Ugly Betty), a ruthless spy who will stop at nothing to get what she needs. Meanwhile, Ellie and Awesome's date night takes a dangerous turn and Morgan is sent on a special mission to recover Chuck's P.A.N.T.S.

Chuck and Sarah discover who is behind the plot to destroy Carmichael Industries. Meanwhile, Ellie's plans for a perfect Christmas begin to come apart and, at the Buy More, Morgan and the gang must fend off angry customers as a computer virus threatens to ruin Christmas. Comic book legend STAN LEE guest stars. and, in return, gets makeover advice.

Sarah is forced to confront a secret from her past and feels she must do so alone, while Chuck urges her to let him help. When Sarah encounters her original handler, Kieran Ryker (guest star TIM DeKAY – White Collar), she fears that he will harm those closest to her, including her mom (guest star CHERYL LADD – Las Vegas, Charlie's Angels).

While Chuck and Sarah contemplate the next step for Carmichael Industries, Casey's forbidden love, Gertrude Verbanski (recurring guest star CARRIE-ANNE MOSS), walks back into his life with a questionable mission in South Beach. Meanwhile, Jeff's enhanced intelligence leads him and Lester to suspect that something unusual may be happening at the Buy More.

As Chuck and Sarah try to realize a new, safer future, Morgan's past as the Intersect sends them to Vail on one last mission, where they enlist the help of an unlikely source - the legendary BO DEREK (guest starring as herself - 10, Fashion House, Tommy Boy). Elsewhere, Jeff and Lester continue their own spy mission. Recurring guest star ANGUS MACFADYEN (Braveheart) appears as Nicholas Quinn.

When a mission in Japan becomes a hostage situation, Sarah and Chuck must face the merciless Nicholas Quinn (recurring guest star ANGUS MACFADYEN) aboard a speeding Japanese bullet train. Meanwhile, the mission leaves Casey with an impossible decision.

After a harrowing mission, Sarah returns to Chuck with a huge secret. Meanwhile, Ellie and Awesome are presented with a new opportunity that could change their lives. Recurring guest star ANGUS MACFADYEN appears as Nicholas Quinn.

In the series finale, Chuck enlists his family, friends and some unexpected allies as he races to stop Nicholas Quinn (recurring guest star ANGUS MACFADYEN) from destroying everything Chuck has built over the past five years. Recurring guest star LINDA HAMILTON appears as Mary Bartowski.