When Commissioner Gordon is framed for taking bribes from Rupert Thorne, his daughter Barbara pleads with Batman to show up at a rally being put on in the commissioner's behalf. But when Batman disappears after finding the person behind the frame-up, Barbara takes the law into her own hands as Batgirl.

Robin discovers that Gil Mason is in league with the underworld and goes to investigate him. He encounters Batgirl along the way, and go their separate ways to stop Mason. They then meet again to learn that Gil is working with Two-Face to take out Gordon, and have Batman (as Matches Malone) captured.

A female scientist helps Clayface when his body begins to disintegrate.

Wealthy socialite Veronica Vreeland returns from Central America bringing tiny handmade dolls for all her friends. According to native legend, once placed under a pillow the dolls do the sleeper's worrying for them. Unknown to Veronica or her guests, each of the dolls was made by the Mad Hatter, and contains a tiny microchip which plants hypnotic suggestions inside the sleepers' brains.

Dr. Leslie Thompkins is kidnapped when crime boss Rupert Thorne requires surgery.

Poison Ivy appears to have gone straight and become suburban homemaker, nevertheless Batman suspects her of a series of plant based crimes in Gotham City.

When pursuing the escaped convict Killer Croc, Batman encounters a small group of sideshow freaks who apparently trust Croc more than Batman.

Batman must stop Ra's Al Ghul when he attempts to unleash the power of the ancient God Thoth Khepera.

Batman's worst enemies kidnap him and put him on trial for crimes committed against them.

The Joker steals an atomic bomb and Batman and Robin must team up with Harley Quinn in order to find him.